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Sussan TE, Gajghate S, Thimmulappa RK, Ma J, Kim J-H, Sudini K, Consolini N, Cormier SA, Lomnicki S, Hasan F, Pekosz A, Biswal S (2015) Exposure to electronic cigarettes impairs pulmonary anti-bacterial and anti-viral defenses in a mouse model

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Key facts about vaping There is no combustion, which means no tar Vapour contains only the ingredients in the e-liquid You control the nicotine level Disposable vapes offer a simple starting point Pod kits provide consistency and ease Refillable kits give the most flexibility Vaping is not risk-free, but it is very different from smoking because smoke is not present

Total RNAs were isolated from the cells using miRNeasy kit (Qiagen) for RNA -Seq analysis